Human Immune Monitoring Core (HIMC)

Technology Platforms & Services

The Human Immune Monitoring Core (HIMC) provides state-of-the-art technology platforms and top-notch services for single-cell, in situ, and multiomic analysis required by today's immunology research and clinical studies to help you make impactful discoveries and develop improved treatments for human diseases. 

Spectral flow cytometry (Cytek Aurora)

High-parametric cytometry enabled by the innovative 5-laser, 64-channel spectral cytometer Cytek Aurora, capable of 40+ colors using pre-optimized panels, or ~20-30 colors for routine experiments. It is available by reservation 24/7 to trained users. Assisted usage will also be available during normal business hours in near future when the dedicated technician is hired. 

• Self-operation: trained users have access to the instrument 24/7. Reservation on iLab is required.
• Assisted use & full services: from assisted data acquisition to sample prep & complete experimental workflows (launching in near future). 
• Training: comprehensive training on spectral cytometry concepts, experimental design & optimization, and Aurora operation. Start by emailing Julia Milczanowski for a training request at jum4024@med.cornell.edu. 
• Consultation: for experimental design, troubleshooting, and data analysis strategies or methods. The first 30 min is free for each WCM study. Request on iLab or email Amanda Hennemann at aph4002@med.cornell.edu.

Imaging mass cytometry (Fluidigm Hyperion) 

The Fluidigm Hyperion imaging mass cytometry (IMC) system enables highly multiplexed tissue imaging at up to ~40 markers per panel with minimal spillover between channels using metal-tagged antibodies and at 1 µm/pixel resolution to generate images comparable to light microscopy. This allows high-content, deep profiling of the complex tissue immune microenvironments (TIME) with spatial and organizational information, for important insights into the functional states of individual cells and their interactions in the tissue and disease context.

• Initiate an IMC/Hyperion project or contact Hiranmayi Ravichandran at hir4001@med.cornell.edu. 

CyTOF mass cytometry (Fluidigm Helios)

High-parametric cytometry/single-cell analysis with 40+ markers per panel using metal-tagged antibodies with minimal spillover between channels and no interference from autofluorescence. 

• Initiate a CyTOF/Helios project or contact Hiranmayi Ravichandran at hir4001@med.cornell.edu.

Staff & Oversight
Hiranmayi Ravichandran | Mass Cytometry Specialist | hir4001@med.cornell.edu  

Location & Hours

Hyperion IMC & Helios CyTOF

• 413 E 69th St BB-1676, New York, NY 10021
• Monday-Friday, 10 am-6 pm (by appointment only)

Aurora

• 413 E 69th St BB-950F, New York, NY 10021
• Assisted usage, training, consultation: Monday-Friday, 10 am-6 pm (by appointment only)
• Unassisted self-operation: 24/7 (trained users only)

Links & Resources

1. Weill Cornell Englander Institute for Precision Medicine - Mass Cytometry Website
2. HIMC Aurora & Spectral Cytometry Info

Contacts

Name	Role	Phone	Email	Location
Hiranmayi Ravichandran, MS	Mass Cytometry Specialist		hir4001@med.cornell.edu	BRB-1676
Niroshana Anandasabapathy			nia9069@med.cornell.edu
Julia Milczanowski	Cytek Aurora Technician		jum4024@med.cornell.edu	BRB-952H

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