WCM Proteomics and Metabolomics Core Facility

News

We are hiring! Research Specialist-Proteomics/Metabolomics.
Mar 2019 Paper published with Dr Ting in ACS Chem Neurosci. Real-Time, in Vivo Correlation of Molecular Structure with Drug Distribution in the Brain Striatum Following Convection Enhanced Delivery. (https://pubs.acs.org/doi/10.1021/acschemneuro.8b00607)
Jan 2019 Paper published with Dr Cantley in Cancers. Rac-Mediated Macropinocytosis of Extracellular Protein Promotes Glucose Independence in Non-Small Cell Lung Cancer. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356657/)
Jan 2019 paper published with Dr Cantley in Cell. “Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells” (https://linkinghub.elsevier.com/retrieve/pii/S0092-8674(18)31253-4)
More News ...

Services

Established in 2016, the Weill Cornell Medicine (WCM) Meyer Cancer Center Proteomics and Metabolomics Core Facility offers mass spectrometry-based analysis of proteins, peptides, metabolites and other biochemical molecules, to Weill Cornell Medicine users as well as external users from academic institutions and commercial enterprises.

Proteomics

Protein Identification
Protein Quantitation
- Methods include: SILAC, TMT, Label-free, spiked standards, etc.
Post-Translational Modification (PTM)
- Phosphorylation, ubiquitination, acetylation, N/C-term processing, etc.

Metabolomics

Targeted profiling of polar metabolites (Targeted detection of metabolites in a predefined panel, which includes many central metabolites such as glycolysis/TCA intermediates and amino acids. See the list HERE. See a Sample report. )
Stable isotope tracing / flux for polar metabolites (e.g. C13 glucose labeling)

Type 1: Tracing of selected metabolites (Please select metabolites from the LIST to avoid ambiguity.)
Type 2: Full panel tracing (Exhaustive tracing of all detected metabolites. No need to know beforehand if a particular metabolite is labeled or not. Note: minimum 5 samples)

Thyroid hormones triiodothyronine/thyroxine (T3/T4) absolute quantitation (100 ul serum is needed)
Targeted analysis of 7 polyamines (See the list HERE.)
Accurate MW measurement for drugs/chemicals (mass accuracy: a few ppm)
New method development

Note:

Unless otherwise noted, quantitation in all assays is relative quantitation, not absolute.
Turnaround time is normally 1-2 weeks, except for global protein/PTM profiling (~4 weeks).