About

Proteomics and Metabolomics Core Facility

Notice

  • Current turnaround time: Proteomics ~3 weeks; Metabolomics ~2 weeks. 

Services

Established in 2016, the Weill Cornell Medicine (WCM) Meyer Cancer Center Proteomics and Metabolomics Core Facility offers mass spectrometry-based analysis of proteins, peptides, metabolites and other biochemical molecules, to Weill Cornell Medicine users as well as external users from academic institutions and commercial enterprises.

  • Proteomics
    • Proteomic profiling by label-free quantitation 
      • Label-free quantitation enables straightforward experimental design. 
      • With improved data independend acquisition (DIA) methods, typical number of protein IDs ranges 5,000-7,000 for human/mouse total cell lysate.
      • Popular applications: protein expression profiling; protein interactions (IP); proximity-based labeling for interactome mapping (APEX) etc. 
    • Proteomic and phosphoproteome profiling by TMT quantitation 
      • Stable isotope labeling enables high accuracy in quantitation. Ideal for deep coverage phosphoproteome analysis.
      • Allows comparing up to 18 samples in a single experiment.
      • Typical number of protein IDs 6,000-8,000, phosphosites 10,000 - 20,000 for human/mouse total cell lysate.
      • Popular applications: global phosphoproteomics, etc. 
    • Post-Translational Modification (PTM) analysis
      • Popular applications: PTM characterization of purified protein; histone modifications profiling(See details HERE); crosslinking proteomics, etc.
      • Types of PTM include: Phosphorylation, ubiquitination, acetylation, N/C-term processing, etc.
    • Identification of gel purified proteins
                 Popular applications: confirmation of protein expression, etc.
    • Absolute Quantitation of target proteins 
      • method development is needed (See details HERE)
    • Immunopeptidomics
                 
  • Metabolomics
    • Targeted profiling of polar metabolites  (Targeted detection of metabolites in a predefined panel, which includes many central metabolites such as glycolysis/TCA intermediates and amino acids. See the list HERE. )
    • Untargeted metabolite profiling (See details HERE.)
    • Lipid profiling (See details HERE.)
    • Free fatty acid profiling  (See the list HERE.)
    • Short chain Acyl-CoAs  (See the list HERE.)
    • Nucleoside and nucleotide profiling (See the list HERE.)
    • Bile Acids (See the list HERE.)
    • AcylCarnitines (See the list HERE)
    • Stable isotope tracing
      • For polar metabolites (C13 or N15 labeling. C13-N15 dual labeling only available for selected metabolites. See a Sample report. )
        • Type 1: Tracing of selected metabolites (Please select metabolites from the LIST to avoid ambiguity.)
        • Type 2: Full panel tracing (Exhaustive tracing of all detected metabolites. No need to know beforehand if a particular metabolite is labeled or not.) 
      • For Free Fatty Acids (C13 labeling. Full panel analysis. See the list HERE.)
      • For Lipids (C13 labeling. For selected lipids only. Full panel tracing not available.Based on the Lipid profiling assay.)

       Notes: 

    • Unless otherwise noted, quantitation in all assays is relative, not absolute quantitation.
    • For quantitative analysis, please submit all samples together to minimize variation. It is difficult to compare data acquired at different times.
    • Please note that a minimum of six samples is required for all metabolomics assays.
    • Turnaround time is normally within 10 business days, except for TMT global protein/PTM profiling (~4 weeks). Turnaround time changes from time to time. Please see the NOTICE on the top of the webpage for updates.
    • Standard statistical analysis (such as heatmap, PCA, t-test, one-way anova) may be included in the report for free when appropriate. Additional customized data analysis requests will incur charges. Free software tools are available to avoid such charges (e.g. Metaboanalyst for metabolomics, Perseus for proteomics).

What's in the Report

Sample Preparation Protocols

 

Rates

  • Click HERE to see pricing for the current Fiscal Year
  • Note: service fees are adjusted annually to reflect changes in running cost

Service Request Workflow

  1. Create a service request on iLab
  2. Get a quote from the core
  3. Approve the cost in iLab
  4. Financial approval in iLab by PI or a designated financial admin / lab manager
  5. Drop off samples to the core (see instruction below; by email appointment only; after finacial approval only)
  6. Sample analysis by the core
  7. Receive analysis results
  8. Receive invoices
  9. Payment (external users pay with PO; internal users with WCM funds)

Note: All service requests are done via the online iLab system. An iLab account is needed for sample submissions.

Instrumentation

             Orbitrap Fusion Lumos

    Orbitrap Q Exactive / Vanquish UPLC 

Links and Resources

  • To acknowledge the core in a publication
    • Mass spectrometric analysis was (analyses were) performed at the Weill Cornell Medicine Proteomics and Metabolomics Core Facility.
  • Report format

Contact Us

Director
Guoan Zhang PhD
guz2004 [at] med.cornell.edu
Phone (646) 962-6222

Staff
Dr. Mengmeng Zhu - mez2005 [at] med.cornell.edu
Dr. Xiaoyuan Yang - xiy4003 [at] med.cornell.edu
Dr. Zhucui Li - zhl4007 [at] med.cornell.edu
Dr. Lei Lei - lel4009 [at] med.cornell.edu
Phone (646) 962-9739

Sample Drop off

Samples can be dropped off to Lab 1552 of Belfer building between 10:30am - 4pm. Let the staff know your drop off time (please be specific with the time) in advance by email. Call 646-962-9739 to get into the glass door when arriving at 15th floor. 

Note:  for all sample drop-off, please schedule a time in advance with core staff by email.

Shipping Address

413 East 69th Street, BRB 1552 (Zhang lab)
New York, NY 10021
Lab phone: 646-962-9739

(Please use overnight shipping on dry ice and notify the core of the trackinng number.)