WCM Proteomics and Metabolomics Core Facility

News

Services

Established in 2016, the Weill Cornell Medicine (WCM) Meyer Cancer Center Proteomics and Metabolomics Core Facility offers mass spectrometry-based analysis of proteins, peptides, metabolites and other biochemical molecules, to Weill Cornell Medicine users as well as external users from academic institutions and commercial enterprises.

  • Proteomics
    • Protein Identification
    • Protein Quantitation
      • Methods include: SILAC, TMT, Label-free, spiked standards, etc.
    • Post-Translational Modification (PTM)
      • Phosphorylation, ubiquitination, acetylation, N/C-term processing, etc.
  • Metabolomics
    • Targeted profiling of polar metabolites (Targeted detection of metabolites in a predefined panel, which includes many central metabolites such as glycolysis/TCA intermediates and amino acids. See the list HERE. See a Sample report. )
    • Stable isotope tracing / flux for polar metabolites (e.g. C13 glucose labeling)
      • Type 1: Tracing of selected metabolites (Please select metabolites from the LIST to avoid ambiguity.)
      • Type 2: Full panel tracing (Exhaustive tracing of all detected metabolites. No need to know beforehand if a particular metabolite is labeled or not. Note: minimum 5 samples) 
    • Thyroid hormones triiodothyronine/thyroxine (T3/T4) absolute quantitation (100 ul serum is needed)
    • Targeted analysis of 7 polyamines (See the list HERE.)
    • Accurate MW measurement for drugs/chemicals (mass accuracy: a few ppm)
    • New method development

       Note: 

    • Unless otherwise noted, quantitation in all assays is relative quantitation, not absolute.
    • Turnaround time is normally 1-2 weeks, except for global protein/PTM profiling (~4 weeks).

Fees

  • Click HERE to see pricing
  • Note: service rates are adjusted annually to reflect changes in running cost

Sample Submission / Service Request

Instrumentation

Lumos

             Orbitrap Fusion Lumos

    Orbitrap Q Exactive / Vanquish UPLC

  • Easy-nLC 1200 UPLC 
  • Agilent 1260 

Links and Resources

  • Report format

Contact Us

Director
Guoan Zhang PhD
guz2004@med.cornell.edu
(646) 962-6222

Core Staff
Dr. Mengmeng Zhu - mez2005@med.cornell.edu
Dr. Xiaoyuan Yang - xiy4003@med.cornell.edu

(646) 962-9739

Location / Shipping Address

413 East 69th Street, BRB 1552 (Zhang lab)
New York, NY 10021
Lab phone: 646-962-9739

(For sample drop off, please drop off to Lab 1552 of Belfer building. Call 646-962-9739 to get into the glass door when arriving at 15th floor.)