WCM Proteomics and Metabolomics Core Facility

News

Note: due to the pandemic, for sample drop-off, please schedule a time in advance with core staff to ensure that someone will be in the lab.
Dec 2020 New services available: folate metabolite profiling
Jan 2020 New services available: Untargeted metabolite profiling.
Oct 2019 New services available for lipids, fatty acids, bile acids, acyl-carnitines and short chain acyl-CoAs.
More News ...

Services

Established in 2016, the Weill Cornell Medicine (WCM) Meyer Cancer Center Proteomics and Metabolomics Core Facility offers mass spectrometry-based analysis of proteins, peptides, metabolites and other biochemical molecules, to Weill Cornell Medicine users as well as external users from academic institutions and commercial enterprises.

Proteomics

Protein Identification

protein in gel / in solution

Protein Quantitation
- Popular applications: protein expression profiling; protein interactions (IP); proximity-based labeling for interactome mapping (APEX); Thermo Proteome Profiling (TPP)/Cellular Thermal Shift Assay (CETSA); etc.
- Methods include: SILAC, TMT, Label-free, spiked standards, etc.
Post-Translational Modification (PTM)
- Popular applications: protein phosphorylation profiling; PTM characterization of purified protein; histone modifications; etc.
- PTMs include: Phosphorylation, ubiquitination, acetylation, N/C-term processing, etc.

Metabolomics

Targeted profiling of polar metabolites (Targeted detection of metabolites in a predefined panel, which includes many central metabolites such as glycolysis/TCA intermediates and amino acids. See the list HERE. See a Sample report. )
Stable isotope tracing / flux for polar metabolites (e.g. C13 glucose labeling. See a Sample report. )

Type 1: Tracing of selected metabolites (Please select metabolites from the LIST to avoid ambiguity.)
Type 2: Full panel tracing (Exhaustive tracing of all detected metabolites. No need to know beforehand if a particular metabolite is labeled or not. Note: minimum 6 samples)

Untargeted metabolite profiling (See details HERE.)

Lipid profiling (See the list HERE.)
Free fatty acid profiling (See the list HERE.)
Bile acids (See the list HERE.)
Acylcarnitine profiling (See the list HERE.)
Short chain Acyl-CoAs (See the list HERE.)
Folate metabolites (See the list HERE.)
Thyroid hormones triiodothyronine/thyroxine (T3/T4) absolute quantitation (100 ul serum is needed)
Targeted analysis of 7 polyamines (See the list HERE.)
Accurate MW measurement for drugs/chemicals (mass accuracy: a few ppm)
New method development

Notes:

Unless otherwise noted, quantitation in all assays is relative quantitation, not absolute.
Turnaround time is normally 5-10 business days, except for global protein/PTM profiling (~4 weeks).