WCM Proteomics and Metabolomics Core Facility

News

• Note: due to the pandemic, for sample drop-off, please schedule a time in advance with core staff to ensure that someone will be in the lab.
• Dec 2020 New services available: folate metabolite profiling 
• Jan 2020 New services available: Untargeted metabolite profiling.
• Oct 2019 New services available for lipids, fatty acids, bile acids, acyl-carnitines and short chain acyl-CoAs.
• More News ... 

Services

Established in 2016, the Weill Cornell Medicine (WCM) Meyer Cancer Center Proteomics and Metabolomics Core Facility offers mass spectrometry-based analysis of proteins, peptides, metabolites and other biochemical molecules, to Weill Cornell Medicine users as well as external users from academic institutions and commercial enterprises.

• Proteomics
• Protein Identification
• protein in gel / in solution
• Protein Quantitation
  • Popular applications: protein expression profiling; protein interactions (IP); proximity-based labeling for interactome mapping (APEX); Thermo Proteome Profiling (TPP)/Cellular Thermal Shift Assay (CETSA); Targeted Protein Degradation (TPP), etc. 
  • Methods include: SILAC, TMT (See details HERE), Label-free, spiked standards, etc.
• Post-Translational Modification (PTM)
  • Popular applications: protein phosphorylation profiling; PTM characterization of purified protein; histone modifications profiling(See details HERE); crosslinking proteomics, etc.
  • PTMs include: Phosphorylation, ubiquitination, acetylation, N/C-term processing, etc.

• Metabolomics
• Targeted profiling of polar metabolites (Targeted detection of metabolites in a predefined panel, which includes many central metabolites such as glycolysis/TCA intermediates and amino acids. See the list HERE. See a Sample report. )
• Stable isotope tracing / flux for polar metabolites (e.g. C13 glucose labeling. See a Sample report. )
• Type 1: Tracing of selected metabolites (Please select metabolites from the LIST to avoid ambiguity.)
• Type 2: Full panel tracing (Exhaustive tracing of all detected metabolites. No need to know beforehand if a particular metabolite is labeled or not. Note: minimum 6 samples) 

• Untargeted metabolite profiling (See details HERE.)

• Lipid profiling (See the list HERE.)
• Free fatty acid profiling  (See the list HERE.)
• Bile acids  (See the list HERE.)
• Acylcarnitine profiling  (See the list HERE.)
• Short chain Acyl-CoAs  (See the list HERE.)
• Folate metabolites  (See the list HERE.)
• Thyroid hormones triiodothyronine/thyroxine (T3/T4) absolute quantitation (100 ul serum is needed)
• Targeted analysis of 7 polyamines (See the list HERE.)
• Accurate MW measurement (HRMS) for drugs/chemicals (mass accuracy: a few ppm)
• New method development

       Notes: 

• Unless otherwise noted, quantitation in all assays is relative quantitation, not absolute.
• Turnaround time is normally 5-10 business days, except for global protein/PTM profiling (~4 weeks).

Sample Preparation Protocols

• Proteomics.

• Polar metabolites.
• Lipids.
• Free fatty acids.
• Bile acids.
• Short Chain Acyl-CoAs.
• Folates.
• Acylcarnitines.
• Polyamines.
• HRMS.

Fees

• Click HERE to see pricing
• Note: service rates are adjusted annually to reflect changes in running cost

Service Request

Note: All service requests are done via the online iLab system. An iLab account is needed for sample submissions.

• How to create a Service request by External users, Internal users and PIs.
• Payment instructions.
• Accessing iLab from MSKCC.

Instrumentation

             Orbitrap Fusion Lumos

    Orbitrap Q Exactive / Vanquish UPLC

• Easy-nLC 1200 UPLC 
• Agilent 1260 

Bruker timsTOF Pro 2

• High sensitivity mass spectrometer for label-free proteomics

Links and Resources

• To acknowledge the core in a publication
• Mass spectrometric analysis was (analyses were) performed at the Weill Cornell Medicine Proteomics and Metabolomics Core Facility.
• Report format

• Proteomics report format
• recommended free online tool for metabolomics data analysis
• Metaboanalyst

Contact Us

Director
Guoan Zhang PhD
guz2004@med.cornell.edu
Phone (646) 962-6222

Staff
Dr. Mengmeng Zhu - mez2005@med.cornell.edu
Dr. Xiaoyuan Yang - xiy4003@med.cornell.edu
Phone (646) 962-9739

Sample Drop-off / Shipping Address

413 East 69th Street, BRB 1552 (Zhang lab)
New York, NY 10021
Lab phone: 646-962-9739

(For sample drop off, please drop off to Lab 1552 of Belfer building. Call 646-962-9739 to get into the glass door when arriving at 15th floor.)

Note: due to the pandemic, for all sample drop-off, please schedule a time in advance with core staff to ensure that some one will be in the lab.