WCM Proteomics and Metabolomics Core Facility

News

• Mar 2019 Paper published with Dr Ting in ACS Chem Neurosci. Real-Time, in Vivo Correlation of Molecular Structure with Drug Distribution in the Brain Striatum Following Convection Enhanced Delivery. (https://pubs.acs.org/doi/10.1021/acschemneuro.8b00607)
• Jan 2019 Paper published with Dr Cantley in Cancers. Rac-Mediated Macropinocytosis of Extracellular Protein Promotes Glucose Independence in Non-Small Cell Lung Cancer. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356657/)
• Jan 2019 paper published with Dr Cantley in Cell. “Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells” (https://linkinghub.elsevier.com/retrieve/pii/S0092-8674(18)31253-4)
• More News ... 

Overview of Services

Established in 2016, the Weill Cornell Medicine (WCM) Meyer Cancer Center Proteomics and Metabolomics Core Facility provides mass spectrometry based analysis of proteins, peptides, metabolites and other biochemical molecules.

The core facility is open to Weill Cornell Medicine users as well as external users from academic institutions and commercial enterprises.

Services

• Proteomics
• Protein Identification
• Protein Quantitation
  • Methods include: SILAC, TMT, Label-free, spiked standards, etc.
• Post-Translational Modification (PTM)
  • Phosphorylation, ubiquitination, acetylation, N/C-term processing, etc.

• Metabolomics
• Targeted profiling of polar metabolites (Targeted detection of metabolites in a predefined panel, which includes many central metabolites such as glycolysis/TCA intermediates and amino acids. See the list HERE.)
• Stable isotope tracing / flux for polar metabolites (e.g. C13 glucose labeling)
• Type 1: Tracing of selected metabolites (Up to 10 metabolites from the panel can be specified. Please use compound names from the LIST to avoid ambiguity.)
• Type 2: Full panel tracing (Exhaustive tracing of all detected metabolites. No need to know beforehand if a particular metabolite is labeled or not.) 
• New! Thyroid hormones triiodothyronine/thyroxine (T3/T4) absolute quantitation (100 ul serum is needed)
• New! Targeted analysis of 7 polyamines (See the list HERE.)
• New method development

       Note:  Unless otherwise noted, quantitation in all assays is relative quantitation, not absolute.

Fees 

Click HERE to see pricing

Sample submission 

Please follow instructions in "Links and Resources" section below. An iLab account is needed to request services. 

Instrumentation

             Orbitrap Fusion Lumos

    Orbitrap Q Exactive / Vanquish UPLC

• Easy-nLC 1200 UPLC 
• Agilent 1260 

Links and Resources

• iLab instructions
• How to create a Service request by External users, Internal users and PIs.
• Payment instructions.
• Seek help from iLab ServiceDesk.
• Accessing iLab from MSKCC.

• Sample Prep Protocols
• Protocols for proteomics.
• Polar metabolites.
• Polyamines.

• Metabolomics targeted panels
• List of polar metabolites for the targeted LC-MS assay.
• List of polyamines for the targeted polyamine assay.

• Report format

• Proteomics report format

Contact us

Director
Guoan Zhang PhD
guz2004@med.cornell.edu
(646) 962-6222

Core Staff
Mengmeng Zhu - mez2005@med.cornell.edu
Xiaoyuan Yang - xiy4003@med.cornell.edu

(646) 962-9739

Location/Shipping Address

413 East 69th Street, BRB 1552 (Zhang lab)
New York, NY 10021
Lab phone: 646-962-9739

(For sample drop off, please drop off to Lab 1552 of Belfer building. Call 646-962-9739 to get into the glass door when arriving at 15th floor.)