About

Sunset - Mouse Genetics Core - Transgenic Core (WCMC)

Overview of Services

 

The role of the Mouse Genetics Core Facility (MGCF) is to facilitate the use of mouse molecular genetics for investigators at MSKCC and neighboring institutions, including WCMC, for in vivo studies of gene functions. The expertise of MGCF covers 3 broad technical areas for the generation and utilization of genetically engineered mice (GEM), namely molecular and cell biology, embryo manipulation, and reproductive biology. As an integral role of the MGCF, staff members regularly engage with investigators in consultation, discussion, and training in the design and production of GEM models, ES cell manipulation, and GEM  breeding and genetics. In addition, MGCF continues to pursue and implement new technical developments that will expedite GEM generation. Much of MGCF's recent effort has been devoted to technical advancements in genome editing using CRISPR/Cas9 system and in ES cell approaches to generate mice with complex genetic modifications, which will contribute to the productivity of WCM investigators.

Hours of Operation

 

Mon - Fri, 9am - 5pm ET

 

Links and Resources

        Mouse Genetics Core Facility Website

        http://onemsk/ski/MouseGenCF/Pages/MGCFHome.aspx

        Jax Website

http://www.jax.org/

 

 

 

Services

 

Molecular and Cell Biology

  • Design and preparation of molecular reagents, such as transgene DNA and genome editing cocktails
  • Genotyping of founder mice
  • Identification and verification of alleles carried by produced GEM models
  • Culture and manipulation of mouse embryonic stem (ES) cells
  • Gene targeting in ES cells to generate new mutant alleles
  • Establishment of ES cells from GEM lines.



Embryo Manipulation

  • Micromanipulation of zygotes to introduce transgene DNA and genome editing tools, such as CRISPR/Cas9
  • Microinjection of ES cells into pre-implantation host embryos

 

Reproductive Biology

  • Strain rederivation or recovery through IVF and/or embryo transfer
  • Cryopreservation and long-term storage of GEM lines
  • Performance of special embryological and animal surgical procedures
  • Provision of GEM resources, including fluorescent reporter mice, CRE and FLPe recombinase mice, and Cas9 mice