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Mouse Genetics Core - Transgenic Core (WCMC)

Overview of Services

 

The primary role of the Mouse Genetics Core Facility (MGCF) is to facilitate the use of mouse molecular genetics at Memorial Sloan-Kettering Cancer Center for in vivo studies of gene functions germane to cancer. Biological problems investigated using the services and expertise of the MGCF include cell survival and proliferation, embryonic development and cell differentiation, organogenesis, cell-cell interactions, immune responses, and the generation of mouse models of cancer. The MGCF consists of three interacting groups: the Transgenic/Embryology Group (TgG), the Tissue Culture Group (TCG), and the Colony Management Group (CMG). The MGCF performs work both at the Long Island City facility and on the main campus in Manhattan.

Hours of Operation

 

Mon - Fri, 9am - 5pm ET

 

Links and Resources

 

http://www.jax.org/

 

Services

 

The Transgenic/Embryology Mouse Group

  1. Purification of transgene DNA
  2. Production of transgenic mice
  3. Genotyping of transgenic founders
  4. Injection of ES cells into blastocysts
  5. Chimera Breeding
  6. Cryopreservation
  7. Rederivation
  8. Special procedures
  9. Provision of special strains



The Tissue Culture Group

  1. Assist users in all aspects of ES cell culture, gene targeting, and ES cell establishment
  2. Provision of mouse embryonic fibroblasts (MEF) and ES cell stocks

The Colony Management Group

  1. General husbandry service
  2. Comprehensive Colony Management