Automated Optical Microscopy

Overview

The Microscopy & Image Analysis Core/Automated Optical Microscopy Services (Formerly the Cell Screening Core) provides equipment and expertise to assist investigators in performing high-content image-based analysis of cells in multi-well plates. With high-content analysis, quantitative phenotypic measurements can be made on individual cells. Phenotypic changes may include changes in the production of cellular products such as proteins and changes in the morphology of cells and their components.  Multiple parameters can be measured simultaneously for each cell. Imaging can be done in standard 6, 24, 96, 384 & 1536-well plates.

Large scale projects include chemical compound library screening for drug discovery and RNAi library screening for target identification and validation. In addition, the equipment can be used for a variety of quantitative imaging projects of any size.

The main proficiencies of Automated Optical Microscopy Services include our flexibility to be able to handle large screens with hundreds of multi-well plates as well as small experiments with only a few wells in one plate and our expertise in being able to design custom analysis algorithms that meet research needs. Researchers here have easy access to their images, data and analysis tools on the WCMC network.

Automated slide scanning of brightfield and fluorescent slides is also available.

 Services

Imaging of Multi-well plates: Imaging is performed on our ImageXpress Micro Confocal High-Content Analysis System

Automated Slide Scanning: Scanning of up to 100 slides per run is performed on our Zeiss Axioscan 7 Slide Digitizer.

Consultation & Training: Consultation on experimental design and assay development. Training can be done for image processing and analysis using MetaXpress software for screening and MetaMorph software for microscopy.

Data Analysis: Processing and analysis of screening images can be performed on our two image processing workstations located in A-232.

Sample Preparation: Preparation of assay plates including dispensing of cells and reagents and plate washing can be performed using the facility equipment located in A-230.

Compound and RNAi Screening: Through the High Throughput Screening Resource Center at Rockefeller University we have access to a library of over 170,000 structurally diverse compounds, natural products and known drugs. RNAi libraries targeting Human, Mouse, Drosophila and C. elegans genomes are available for screening. Seehttp://www.rockefeller.edu/htsrc/ for details.

 Leadership

 

Core Director: Harold "Skip" Ralph, M.P.A.
Core Senior Director: Lee Cohen-Gould, M.S.

 

Location and hours of operation

Hours Location

 

Mon-Fri 10AM-6PM        

 

 

1300 York Ave, Box 63
Rm A-230
New York
NY
10065

 

Links and Resources

  1. https://weillcornellmicroscopy.org/

Contacts

Name Role Phone Email Location
Harold "Skip" Ralph
Co-Director - Microscopy & Image Analysis Core
 
646-962-7726
 
har2001@med.cornell.edu
 
A-230
 

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